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文献和实验stopcock, and all the sterilized parts from step 4.5 onto this sterile field. 7) Now use sterile gloves to connect two 4-way stopcocks. Place caps on the open sample ports. 8) Detach the male and female luer adaptors attaching
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
, contaminating the sterile media. 1. After sterilization and cooldown, increase the airflow until the vessel pressure reaches ~10 psi. 2. Shut down the air service valve at the copper pipe on the wall of the fermentor room. This activates the exhaust
. The first is to Dialyze the coupled antibody against PBS for 24 hours with 3 changes of PBS. Doing this will not change concentration of original antibody (recommended for antibody with a concentration less than 1mg/mL). The second way is to use a PD-10 column. Using
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