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文献和实验by the straight bold line. The liver is right-sided. Modified from ref. 15 . Top right: situs inversus totalis. Heart (arrows), liver, and stomach sidedness are all mirror image of normal. The vertical bar denotes the midline. Courtesy of P. Weinberg. Bottom left
Saturation Analysis of Ligand Binding Using a Centrifugation Procedure
of a typical assay in which unlabeled glycine has been used to displace [3 H]glycine (100 nM) in well‐washed cortical membranes (163 µg/tube protein in this example) from a male NIH‐Swiss mouse. In this representative experiment, the IC50 of glycine was 479 nM
-Use sterile technique and sterile solutions throughout this method.- 1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturation. *** It is important to make sure all strains to be tested are completely
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