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文献和实验Array CGH of Microdissected Fresh or Formalin Fixed DNA
the slide back and forth to spread out the probe mix over the whole array, making sure it reaches all edges of the rubber cement. Sometimes it needs a little help and you can use a pipet tip to gently coach it to the edge. Apply probes to second slide
Screening Colonies by Hybridization with Radiolabeled Probe
Screening Colonies by Hybridization with Radiolabeled Probe Lysis of Colonies and Binding of DNA to Nitrocellulose Filters Labeling of DNA-Probe with 32 P Hybridization to Nitrocellulose
number. In the initial cycles of PCR, there is little change in fluorescence signal. This defines the baseline for the amplification plot. An increase in fluorescence above the baseline indicates the detection of accumulated PCR product. A fixed
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