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文献和实验Real-Time qRT-PCR standard curves... efficiency is too h
into a plasmid and invitro trascripted(IVT) into GAPDH mRNA --> treat with DNase I to completly remove DNA conteminant--> stop DNase reaction --> purify the mRNA --> quantitate you mRNA*-->converte xx ug/ul into xx RNA copy/ul--> perform
High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up
. For more information, see http://www.invitrogen.com or call Technical Service. Follow the protocol below to purify PCR products. The volumes given are on a per sample basis. 1. To ~50 µl PCR samples in 96-well plates, add 10 µl ChargeSwitch® Magnetic Beads.
Differential cDNA Screening Procedures
. Much of the above can be supplied in kit forms by major suppliers, particularly: Stratagene, Promega and BRL. Their tech. service are normally pretty helpful.
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