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文献和实验the diatomaceous earth by centrifugation at 3,000 rpm for 5 minutes in the in the Beckman GPR tabletop centrifuge. 13. Transfer the supernatant to a 1.5 ml microcentrifuge tube and centrifuge at 12,000 rpm for 5 minutes in a microcentrifuge at room temperature
DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
a rack of collection microtubes without samples or buffers, but containing tungsten carbide beads, and fixing this second sandwich into the empty clamp (本方法用来对2×96=192个样本进行提取DNA ,如果希望处理96个或更少样本,要对搅拌磨MM 300进行平衡处理,方法是用一架没有样本和缓冲液但装有碳化钨珠子的收集微型管插入第二个孔板,并用空夹子加入
Replication timing by density transfer
on the frozen azide/EDTA and immediately add the cell sample. Vortex or shake the tube vigorously to chill the sample and break up the frozen EDTA. Spin down the cells in a chilled centrifuge, wash with 1 ml cold water per sample in Eppendorf tubes and freeze
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