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文献和实验Add 700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs
Gene Function Analysis Using the Chicken B-Cell Line DT40
will give a step by step standardized protocol to creating a gene knockout mutant in DT40. With careful consideration, the methods and protocols described herein can be easily modified to allow for further gene manipulations such as creating a knockin
Preparation of 2′‐Deoxy‐2′‐Methylseleno‐Modified Phosphoramidites and RNA
Basic Protocol 3: Deprotection, Purification, and Analysis of RNA Oligonucleotides Containing 2′‐Methylseleno Labels Basic Protocol 4: Enzymatic Ligation of Selenium‐Modified RNA Oligonucleotides Using T4 RNA Ligase
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