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- Beckman Coulter贝克曼库尔特
- 美国
- 001-10091
- 2025年07月13日
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文献和实验Transgenic Outline: Mice and Rats
number, integration site number, and transgene integrity in the transgenic founders prior to breeding. Saunders, 2003. microinjection DNA . Please note, if you want use large DNA fragments such as bacterial artificial chromosomes
Transgenic Mouse and Transgenic Rat Outline
founders. You also need to have a Southern blot assay so that you can determine the copy number, integration site number, and transgene integrity in the transgenic founders prior to breeding. 5. Establish an Expression Assay. It's important
Gene Knockout In Murine Embryonic Stem (ES) Cells
can also be stripped and probed with a neo probe to check for single integration events these are preferable but not essential. Expansion of positive clones Once positive clones have been identified they need to be expanded and stored in liquid nitrogen
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