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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见瓶身
- 保质期:
/
- 英文名:
/
- 库存:
999
- 供应商:
麦格生物
- CAS号:
/
- 规格:
12 kg
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文献和实验Acid-guanidinium thiocyanate-phenol-chloroform RNA purification
with ribonucleases. This method describes preparation from small quantities (~ 50mg) of tissue. Appropriate scaling of the volumes involved can be performed to accomodate larger quantities. You will need: Guanidine isothiocyanate (Sigma) 1M sodium
Assessment of Cyclooxygenase RNA Expression by Northern Hybridization
and tissues are homogenized in a denaturing solution containing 4 M guanidine thiocyanate. The homogenate is mixed sequentially with 2 M sodium acetate (pH 4.0), phenol, and finally chloroform/isoamyl alcohol or bromochloropropane (3 ). The resulting mixture
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
Tris-Cl 6g Water to 2 L, pH 7.4-7.5 1xTRIzol: 200ml Final Add Guanidine thiocyanate 0.8 M FW 118.2 18.912 g Ammonium thiocyanate 0.4 M FW 76.12 6.09 g Sodium Acetate 0.1 M, pH 5 Stock 1M 20 ml Glycerol 5% 10ml Add water to 100ml Mix
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