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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见瓶身
- 保质期:
/
- 英文名:
/
- 库存:
999
- 供应商:
麦格生物
- CAS号:
/
- 规格:
500 ml
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文献和实验g. Wash & Lysis: PE: 10 mM PIPES, 1mM EDTA, 8 mM BME Make a 50X stock and pH to 7.2 with KOH LB: 1mM Tris-HCl, 8 mM BME Make Tris as 2M stock and pH to 8.0 with HCl LB + 0.5% NP-40 : (warm for 30' to 37 deg.C
tubes (cold) Solutions: A lot of these solutions are w/w; Tim says that to make these weigh out sucrose and then add buffer till weight is 100g. Wash & Lysis: PE: 10 mM PIPES, 1mM EDTA, 8 mM BME Make a 50X stock
Preparation of Bacterial Proteins for Analysis by 2D-PAGE
to the bacteria under study the following buffers are used. 2D Lysis Buffer 0.01M Tris-HCl, pH7.4 1mM EDTA 8M Urea 0.05M DTT 10% (v/v) glycerol 5%(v/v) NP40 6% (w/v) ampholytes The buffer is made up using high purity MilliQ
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