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Aβ-IN-8
MCE 国际站:Aβ-IN-8
产品活性:Aβ-IN-8 (compound 7e) 是 Aβ 聚集的有效抑制剂。
研究领域:Neuronal Signaling
作用靶点:Amyloid-β
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文献和实验天进行细胞造模,开始实验。 也可以用PC12细胞株,常规培养 Aβ产物诱导海马神经细胞,建立细胞模型: 选择Aβ25-35片段,用三蒸水配制成100μmol•L-1,过滤,分装,-20℃冻存;使用前老化处理并配制成所需浓度。(将Aβ25-35溶解在DMSO中,在用DMEM稀释,置于37℃下孵育7-14天,即为老化状态)。 细胞模型建立:加入浓度为20µmol/L的Aβ25-35,接触24h,诱导建立AD细胞模型. 检测指标:MTT LDH 凋亡 细胞
Electrophoretic Separation and Immunoblotting of Aβ1–40and Aβ1–42
The main protein component of the plaques found in the brains of Alzheimer’s disease patients is Aβ, a peptide of 39 to 43 amino acids (reviewed in refs. 1 and 2). Two major Aβ isoforms have been identified in the brains of affected individuals
Quantifying Aβ1–40 and Aβ1–42 Using Sandwich-ELISA
The role of Aβ accumulation in the pathogenesis of Alzheimer’s disease (AD) is supported by genetic studies showing that mutations in the amyloid-β precursor protein (APP) that alter Aβ production are linked to a subset of familial AD (FAD) cases
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