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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
868
- 靶点:
CD40
- 级别:
CD40 Antibody
- 目录编号:
CD40 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD40 Antibody
- 抗体英文名:
Dazzle™ 594 anti-mouse CD40 Antibody-PE
- 抗体名:
CD40 Antibody
- 标记物:
PE
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD40 Antibody
- 亚型:
CD40 Antibody
- 形态:
CD40 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2400.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of thetumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelialcells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switchingand, in combination with other signals such as IL-4, protects B cells from surface Ig-inducedapoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), whichis expressed on activated T cells, is important in costimulation and immune regulation.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Recombinant mouse CD40 protein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
2. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations:
1. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
2. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
3. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
原装进口荧光二抗,包括Jackson、KPL的大包装粉末形式的 Dylight荧光二抗,另外,也提供EarthOx的小包装液体形式Dylight荧光二抗,均具有很高的性价比。针对荧光标记二抗,有FITC、 TRITC、Rhodamine、PE、Cy3/5、AMCA、Dylight等多种荧光标记二抗;按照检测物种分,有抗 人、大鼠、小鼠、猪、驴、绵羊、山羊、小鸡、马、兔、仓鼠、狗、牛,以及多种细菌类二抗;另外,还有IgG、IgA、IgM以及IgG(Fc)、 IgG(ab’)2等不同类型抗体
, Rhodamine Red等568nm处红色荧光染料。 CF 568染料耐光性最好;高效水溶性;比Alexa Fluor 568 标记的抗体亮度更亮。 CF 594、Alexa Fluor® 594, ATTO™ 594, DyLight™ 594, Texas Red 等最亮红色荧光染料。 CF594由于其高量子产量和优异的水溶性而比AlexaFluor594 明亮2~4倍。同时CF594 对光极其稳定,使它能被理想地应用于诸如共聚焦显微镜和单分子显像条件苛刻的应用中去。 5、远红外











