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CREB3L4 Polyclonal Antibody（WB/IHC）
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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 供应商：
赛默飞世尔科技
- 抗原来源：
Recombinant protein corresponding to Human CREB3L4
- 抗体名：
CREB3L4
- 宿主：
Rabbit
- 适应物种：
Human
- 亚型：
IgG
- 保存条件：
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- 规格：
100 µL
CREB3L4 Polyclonal Antibody
产品详细信息
Immunogen sequence: ELERHNISLV AQLRQLQTLI AQTSNKAAQT STCVLILLFS LALIILPSFS PFQSRPEAGS EDYQPHGVTS RNILTHKDVT ENLETQVVES RLREPPGAKD ANGSTR
Highest antigen sequence identity to the following orthologs: Mouse - 72%, Rat - 71%.
靶标信息
cAMP response element-binding (CREB) proteins are a familyof mammalian transcription activators. For further backgroundinformation on CREB proteins, see CREB1 (MIM 123810).[supplied byOMIM].
CREB3L4 Antibody (PA5-58254) Relative expression in different tissues in IHC: Detection of differential expression levels of CREB3L4 demonstrates antibody specificity. Immunohistochemical analysis of CREB3L4 using anti-CREB3L4 Polyclonal Antibody (Product # PA5-58254), shows significant staining of CREB3L4 in human prostate and shows minimal or weak staining in human pancreas tissues. The relative expression levels of CREB3L4 within each tissue is shown using RNA-Seq. {RE}
CREB3L4 Antibody (PA5-58254) in ICC/IF Immunofluorescent staining of CREB3L4 in human cell line A-431 using a CREB3L4 Polyclonal Antibody (Product # PA5-58254) shows localization to nucleoplasm, nuclear membrane and mitochondria.
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文献和实验Journal of mammary gland biology and neoplasia
The Novel Transcription Factor CREB3L4 Contributes to the Progression of Human Breast Carcinoma.
"PA5-58254 was used in Immunohistochemistry to focus on the importance and relevance of the CREB3 family in BC and to identify which family member is most involved in the development of breast cancer, as well as identify a possible target gene of CREB3L4."
Authors Pu Q,Lu L,Dong K,Geng WW,Lv YR,Gao HD
() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
Polymer二抗进行了反应的放大，该方法敏感性较高，可缩短抗体孵育时间或降低抗体浓度。 （2）内源性过氧化物酶残余：适度延长内源性过氧化物酶阻断剂阻断时间。 （3）一抗导致的非特异性染色：多克隆抗体易于产生非特异性染色，结合抗体官网信息合理选择多克隆抗体或单克隆抗体。 （4）一抗种属来源与组织样本来源相同：如使用鼠源一抗检测小鼠组织，尽量选择种属来源与样本种属不同的一抗。 （5）切片或细胞过于干燥：实验过程中，需保持样本的湿润。 Q5：阳性染色细胞定位不正确，如何修正？ （1）抗原修复条件不合理：尝试改变
清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7.第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克隆抗体。多克隆抗体的抗原专一性较差，非特异性反应较明显，效价不太稳定。但多克隆抗体制备简便，价格低廉，抗体效价较高，稀释度一般在1：100～1000之间，高者可达
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