The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia.The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.
生物安全等级
1
生长培养基
MEM+10% FBS+1%P/S
推荐传代比例
1:3-1:6
推荐换液频率
2~3次/周
倍增时间
~33.6小时 (PubMed=2993532); 34小时 (ATCC)
冻存条件
冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养条件
气相:空气,95%;CO2,5% 温度:37℃
致瘤性
Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.)