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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant N-terminal cytokeratin 18
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow, IP, Activity
- 抗体英文名:
Cytokeratin 18 Antibody [SB38b] (FITC)
- 抗体名:
Cytokeratin 18 Antibody [SB38b] (FITC)
- 规格:
0.1mg
Cytokeratin 18 抗体 [SB38b] (FITC),Cytokeratin 18 Antibody [SB38b] (FITC),Cytokeratin 18 抗体 [SB38b] (FITC),Cytokeratin 18 Antibody [SB38b] (FITC)
产品名称:Cytokeratin 18 抗体 [SB38b] (FITC)-Cytokeratin 18 Antibody [SB38b] (FITC)
产品货号:PSI-99-629-0.1mg
产品规格:0.1mg
背景资料:Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18 is a type I intermediate filament protein that exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia and is often co-expressed with cytokeratin 8. Tissues from the gastrointestinal tract, respiratory tract, and urogenital tract as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
保存建议:Cytokeratin 18 抗体 [SB38b] (FITC)-Cytokeratin 18 Antibody [SB38b] (FITC)Store vial at 2-8℃
应用类型:Flow, IP, Activity
点击:Cytokeratin 18 抗体 [SB38b] (FITC)-Cytokeratin 18 Antibody [SB38b] (FITC)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
烧瓶内,放入冰槽中; 2. 荧光素的准备。按每毫克免疫球蛋白加0.01mg荧光素计算,称取所需的荧光素量,用3%重碳酸钠水溶液溶解; 3. 将上述已准备好的抗体与荧光色素溶液等量混合,充分搅拌,使其在0—4℃冰箱中结合18—24h; 4. 透析。荧光素与球蛋白结合完毕后,将球蛋白的溶液以2500r/min离心20min,除去其中少量的沉淀物后,装入透析袋中再置于烧杯中,在0—4℃下用pH8.0的缓冲盐溶液透析过夜;
试剂: 1. 0.01mol/L pH7.2 PBS配方。将NaCl 18g、Na2HPO4 1.15g、KH2PO4 0.2g 溶于2000ml三蒸水中,调整pH至7.2; 2. 0.5mol/L pH9.0碳酸盐缓冲液配法。量取0.5mol/L Na2CO3(5.3%)10ml 加入0.5mol/L NaHCO3(4.2%)90ml中,充分混合后,调整pH至9.0; 3. 3%中碳酸钠水溶液的配制。称取1.5g无水正碳酸钠充分溶解于50
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