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- ProSci
- 美国
- PSI-XPS-1018-0.1mL
- 2025年06月28日
- WB
- 兔
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
MEK 1/2 (Ser218/222) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2.
- 保存条件：
taken without freeze/thawing due to the presence of 50% glycerol
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
兔
- 应用范围：
WB
- 抗体英文名：
MEK1 / 2 (phospho Ser218 / Ser222) Antibody
- 抗体名：
MEK1 / 2 (phospho Ser218 / Ser222) Antibody
- 规格：
0.1mL
MEK1 / 2 (phospho Ser218 / Ser222) 抗体，MEK1 / 2 (phospho Ser218 / Ser222) Antibody，MEK1 / 2 (phospho Ser218 / Ser222) 抗体,MEK1 / 2 (phospho Ser218 / Ser222) Antibody
产品名称：MEK1 / 2 (phospho Ser218 / Ser222) 抗体-MEK1 / 2 (phospho Ser218 / Ser222) Antibody
产品货号：PSI-XPS-1018-0.1mL
产品规格：0.1mL
背景资料：MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.
保存建议：MEK1 / 2 (phospho Ser218 / Ser222) 抗体-MEK1 / 2 (phospho Ser218 / Ser222) Antibody taken without freeze/thawing due to the presence of 50% glycerol
应用类型：WB
点击：MEK1 / 2 (phospho Ser218 / Ser222) 抗体-MEK1 / 2 (phospho Ser218 / Ser222) Antibody查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
) which is probably the best choice, in particular for phospho-specific Abs. Good luck! 真爱满行囊 非常感谢楼上的答复，现在明白了。哎，这个抗体现在就不知道怎么能做出结果了。。。。。再试多几次吧。。。 真爱满行囊 非常感谢，因为进实验室没有多久，还是感觉到现在看的文献太少，对这些问题理解太浅，在看文献的过程中，我还是没有办法理解一个问题，那就是gsk
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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