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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human adherent peripheral blood cells
- 保存条件：
Store vial at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
小鼠
- 应用范围：
Flow
- 抗体英文名：
CD107b Antibody [H4B4] (FITC)
- 抗体名：
CD107b Antibody [H4B4] (FITC)
- 规格：
100tests
CD107b 抗体 [H4B4] (FITC)，CD107b Antibody [H4B4] (FITC)，CD107b 抗体 [H4B4] (FITC),CD107b Antibody [H4B4] (FITC)
产品名称：CD107b 抗体 [H4B4] (FITC)-CD107b Antibody [H4B4] (FITC)
产品货号：PSI-99-604-100tests
产品规格：100tests
背景资料：CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains and is a ligand for E-selectin-mediated cell adhesion. CD107b is expressed in lysosomal/endosomal membranes and on the surface of activated platelets and some tumor cell lines including U937 and KG1a. LAMP-2 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential where they may increase adhesion to extracellular matrix and endothelium.
保存建议：CD107b 抗体 [H4B4] (FITC)-CD107b Antibody [H4B4] (FITC)Store vial at 2-8℃
应用类型：Flow
点击：CD107b 抗体 [H4B4] (FITC)-CD107b Antibody [H4B4] (FITC)查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 cells) B. 0.167 ml cells (2 x 106 cells
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
材料： 抗体球蛋白溶液、0.5mol/L pH9.0碳酸盐缓冲液、无菌生理盐水、异硫氰酸荧光素、1%硫柳汞水溶液、三角烧瓶（25—50ml）、冰及冰槽（或1000ml烧杯）、电磁搅拌器、灭菌吸管、透析袋、玻璃棒、棉线及烧杯（500ml）、pH7.2或8.0的0.01mol/L PBS等。 方法与步骤： 1. 抗体的准备。量取适量已知浓度的球蛋白溶液，置入三角烧瓶中，加入生理盐水及碳酸盐缓冲液，使最终球蛋白
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