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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
SW1222 human colorectal cell line
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
Flow
- 抗体英文名:
CD49f Antibody [4F10] (PE)
- 抗体名:
CD49f Antibody [4F10] (PE)
- 规格:
100tests
CD49f 抗体 [4F10] (PE),CD49f Antibody [4F10] (PE),CD49f 抗体 [4F10] (PE),CD49f Antibody [4F10] (PE)
产品名称:CD49f 抗体 [4F10] (PE)-CD49f Antibody [4F10] (PE)
产品货号:PSI-99-549-100tests
产品规格:100tests
背景资料:CD49f is the 125 kDa integrin alpha6 chain which associates noncovalently with the integrin beta1 subunit (CD29) and with the integrin beta4 (CD104) chain to form, respectively, the very late antigen-6 (VLA-6) and alpha6beta4 integrin heterodimers. VLA-6 is expressed on thymocytes, T lymphocytes, monocytes, and platelets. CD49f/CD29 expression is upregulated on activated T lymphocytes and memory T cells. VLA-6 serves as the laminin receptor on platelets, monocytes, and T cells. VLA-6-mediated binding to laminin provides a costimulatory signal to T cells for activation and proliferation.
保存建议:CD49f 抗体 [4F10] (PE)-CD49f Antibody [4F10] (PE)Store vial at 2-8℃
应用类型:Flow
点击:CD49f 抗体 [4F10] (PE)-CD49f Antibody [4F10] (PE)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
抗[E-AB-F0997A]进行封闭:往 100 μL(细胞数1×106)细胞悬液中加入 1 μg 纯抗,室温封闭 15 min,直接加入流式抗体进行后续实验。 巨噬细胞检测应尽量避免使用含花青素的流式抗体(如 PE-Cy7),会增加非特异性染色。若为非巨噬细胞检测,使用含花青素的流式抗体(如 PE-Cy7)时也需要封闭。
,那么每个通道随便选出1个荧光素就可以组合成4个颜色。以2跟激光的Calibur为例,Calibur各个荧光素之间可以搭配出16种组合。比如常用的搭配是F I T C( F L 1 )+P E(F L 2)+PerCP(FL3)+APC(FL4), 这个搭配组合可以更改成Alex Fluro(FL1)+PE(FL2)+PerCP(FL3)+APC(FL4), 或者FITC(FL1)+PE(FL2)+PerCP(FL3)+Alex Fluro(FL4),或者FITC(FL1)+PE(FL2)+PE-Cy
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