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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified T cells from human lymph nodes
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
IHC-Fr, Flow, Blocking, IP, Stimulation, Activity
- 抗体英文名:
CD45 Antibody [F10-89-4] (PE-Cy5.5)
- 抗体名:
CD45 Antibody [F10-89-4] (PE-Cy5.5)
- 规格:
100tests
CD45 抗体 [F10-89-4] (PE-Cy5.5),CD45 Antibody [F10-89-4] (PE-Cy5.5),CD45 抗体 [F10-89-4] (PE-Cy5.5),CD45 Antibody [F10-89-4] (PE-Cy5.5)
产品名称:CD45 抗体 [F10-89-4] (PE-Cy5.5)-CD45 Antibody [F10-89-4] (PE-Cy5.5)
产品货号:PSI-99-541-100tests
产品规格:100tests
背景资料:CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.
保存建议:CD45 抗体 [F10-89-4] (PE-Cy5.5)-CD45 Antibody [F10-89-4] (PE-Cy5.5)Store vial at 2-8℃
应用类型:IHC-Fr, Flow, Blocking, IP, Stimulation, Activity
点击:CD45 抗体 [F10-89-4] (PE-Cy5.5)-CD45 Antibody [F10-89-4] (PE-Cy5.5)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750(每次测试 5μL),以及 CD16-FITC(每次测试 20 μL),并在冰上孵育 30 分钟。10. 在 4℃ 条件下,300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集:1. 创建新补偿值。2. 如适用
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