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- ProSci
- 美国
- PSI-99-245-0.1mg
- 2025年07月16日
- Flow
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Chicken bursal lymphocytes
- 保存条件：
Store vial at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
小鼠
- 应用范围：
Flow
- 抗体英文名：
CD45 Antibody [LT40] (PE-Cy5)
- 抗体名：
CD45 Antibody [LT40] (PE-Cy5)
- 规格：
0.1mg
CD45 抗体 [LT40] (PE-Cy5)，CD45 Antibody [LT40] (PE-Cy5)，CD45 抗体 [LT40] (PE-Cy5),CD45 Antibody [LT40] (PE-Cy5)
产品名称：CD45 抗体 [LT40] (PE-Cy5)-CD45 Antibody [LT40] (PE-Cy5)
产品货号：PSI-99-245-0.1mg
产品规格：0.1mg
背景资料：Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45low) of thymocytes. The CD45low population contains exclusively thymocytes with the CD3-CD4-CD8low phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8low to CD4+CD8+ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45low subpopulation provides a suitable marker for the CD3-CD4-CD8low immediate precursors to the CD3-CD4+CD8+ thymocytes.
保存建议：CD45 抗体 [LT40] (PE-Cy5)-CD45 Antibody [LT40] (PE-Cy5)Store vial at 2-8℃
应用类型：Flow
点击：CD45 抗体 [LT40] (PE-Cy5)-CD45 Antibody [LT40] (PE-Cy5)查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750（每次测试 5μL），以及 CD16-FITC（每次测试 20 μL），并在冰上孵育 30 分钟。10. 在 4℃ 条件下，300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集：1. 创建新补偿值。2. 如适用
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