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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse CD19-expressing K562 human erythroleukemia cells
- 保存条件:
Store vial at 2-8℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
大鼠
- 应用范围:
E, WB, ICC
- 抗体英文名:
CD19 Antibody [6D5] (PE-Cy7)
- 抗体名:
CD19 Antibody [6D5] (PE-Cy7)
- 规格:
0.1mg
CD19 抗体 [6D5] (PE-Cy7),CD19 Antibody [6D5] (PE-Cy7),CD19 抗体 [6D5] (PE-Cy7),CD19 Antibody [6D5] (PE-Cy7)
产品名称:CD19 抗体 [6D5] (PE-Cy7)-CD19 Antibody [6D5] (PE-Cy7)
产品货号:PSI-98-666-0.1mg
产品规格:0.1mg
背景资料:CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.
保存建议:CD19 抗体 [6D5] (PE-Cy7)-CD19 Antibody [6D5] (PE-Cy7)Store vial at 2-8℃
应用类型:E, WB, ICC
点击:CD19 抗体 [6D5] (PE-Cy7)-CD19 Antibody [6D5] (PE-Cy7)查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750(每次测试 5μL),以及 CD16-FITC(每次测试 20 μL),并在冰上孵育 30 分钟。10. 在 4℃ 条件下,300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集:1. 创建新补偿值。2. 如适用
: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA
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