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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This ERK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-557 amino acids from the C-terminal region of human ERK4.
- 保存条件:
Store at 4℃ for three months and -20℃, stable for up to one year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
WB, IHC-P
- 抗体英文名:
ERK4 Antibody
- 抗体名:
ERK4 Antibody
- 规格:
400ul
ERK4 抗体,ERK4 Antibody,ERK4 抗体,ERK4 Antibody
产品名称:ERK4 抗体-ERK4 Antibody
产品货号:PSI-62-954-400ul
产品规格:400ul
背景资料:Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.
保存建议:ERK4 抗体-ERK4 AntibodyStore at 4℃ for three months and -20℃, stable for up to one year
应用类型:WB, IHC-P
点击:ERK4 抗体-ERK4 Antibody查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验liuxing0626 各位学长好: 我买的ProteinTECH公司的 ERK1 抗体 western blot 条带有两条,公司的说明书也是两条,请问这是个怎么情况?而且我要做ERK1/2。但是ERK2也是单买的,混起来用的话会不会出来3条带了。 ERK1产品地址http://www.ptglab.com/Products/ERK1-Antibody-11257-1-AP.htm liuxing0626
【求助】(Western Blot) 检测不到P-erk却能检测到erk
好的ECL显色剂,这样即使条带信号弱也可以检测出来。不同厂家的质量差别很大! tife 请教各位高手用磷酸化抗体检测p-ERK怎么选择不同磷酸化位点的抗体?用H2O2刺激细胞,是选择酪氨酸激活的还是丝氨酸激活的p-ERK?公司提供的目录上有202、204等位点磷酸化的,不知道怎么选。谢谢大家的指导! pengwu46 Erk活化需要多个位点的同时磷酸化才能够实现,而且活化过程中磷酸化有一个先后的顺序,很复杂。一般认为
ahuchxq 因为最近作磷酸化的ERK的western ,我用的是Hela 229 细胞,但是比较郁闷的是,这个细胞为什么阳性对照组和没有任何处理的一组都有磷酸化的ERK?是我细胞坏了还是本来这个细胞就是这样?我用Hela 细胞试了下,对照组也有一点,但是明显比阳性对照组弱,293细胞也是。但是就是229细胞没有任何区别,我很郁闷,试了好几次都是这样,因为前期大量实验都是在229细胞上做的,所以不能选其他细胞了。。 请求有经验的大哥大姐指教。。。。不胜
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