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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr298 of Xenopus EphrinB. Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.
- 保存条件:
stored at -20℃ and is stable at -20℃ for at least 1 year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
WB
- 抗体英文名:
EphrinB (phospho Tyr298) Antibody
- 抗体名:
EphrinB (phospho Tyr298) Antibody
- 规格:
0.1mL
EphrinB (phospho Tyr298) 抗体,EphrinB (phospho Tyr298) Antibody,EphrinB (phospho Tyr298) 抗体,EphrinB (phospho Tyr298) Antibody
产品名称:EphrinB (phospho Tyr298) 抗体-EphrinB (phospho Tyr298) Antibody
产品货号:PSI-50-222-0.1mL
产品规格:0.1mL
背景资料:EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).
保存建议:EphrinB (phospho Tyr298) 抗体-EphrinB (phospho Tyr298) Antibody stored at -20℃ and is stable at -20℃ for at least 1 year
应用类型:WB
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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