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- ProSci
- 美国
- PSI-33-311-100ug
- 2025年07月16日
- Flow, IF, WB, IHC-P
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human recombinant protein was used as the immunogen for the p21 antibody.
- 保存条件：
Aliquot and Store at 2-8℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
小鼠
- 应用范围：
Flow, IF, WB, IHC-P
- 抗体英文名：
p21 Antibody [DCS-60.2]
- 抗体名：
p21 Antibody [DCS-60.2]
- 规格：
100ug
p21 抗体 [DCS-60.2]，p21 Antibody [DCS-60.2]，p21 抗体 [DCS-60.2],p21 Antibody [DCS-60.2]
产品名称：p21 抗体 [DCS-60.2]-p21 Antibody [DCS-60.2]
产品货号：PSI-33-311-100ug
产品规格：100ug
背景资料：This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This mAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
保存建议：p21 抗体 [DCS-60.2]-p21 Antibody [DCS-60.2]Aliquot and Store at 2-8℃
应用类型：Flow, IF, WB, IHC-P
点击：p21 抗体 [DCS-60.2]-p21 Antibody [DCS-60.2]查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验-mercaptoethanol to 0.1%, glusulase to 0.05%, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37o C. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts
.Optional: Confirm successful protein transfer by Ponceau Red staining before proceeding to the next step.封闭，抗体孵育和洗膜1. Blocking: Incubate the blot in 3% non-fat milk / PBST or Trident Universal Protein Blocking Reagent for 30-60 minutes at RT.2. Primary
to 0.05, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37oC. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts and pellet as above. Resuspend in 10
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