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NMDA NR2B (phospho Tyr1472) 抗体-NMDA NR2B (phospho Tyr1472) Antibody
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified full-length recombinant Survivin-His is expressed in E.Coli strain BL21 (DE3).The antigen is soluble.
- 保存条件:
stored at -20℃, stable for one year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
E, WB
- 抗体英文名:
Survivin Antibody [2H5H2]
- 抗体名:
Survivin Antibody [2H5H2]
- 规格:
0.1mL
Survivin 抗体 [2H5H2] ,Survivin Antibody [2H5H2] ,Survivin 抗体 [2H5H2] ,Survivin Antibody [2H5H2]
产品名称:Survivin 抗体 [2H5H2] -Survivin Antibody [2H5H2]
产品货号:PSI-32-222-0.1mL
产品规格:0.1mL
背景资料:Baculoviral IAP repeat-containing 5 (survivin).This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
保存建议:Survivin 抗体 [2H5H2] -Survivin Antibody [2H5H2] stored at -20℃, stable for one year
应用类型:E, WB
点击:Survivin 抗体 [2H5H2] -Survivin Antibody [2H5H2] 查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
后,将EP管置于磁性分离架上,去除上清,加入封闭液,此时,磁珠的终浓度为5mg/mL,同样置于37℃环境旋转混匀反应1h。 2.5. 封闭完成后,重复2.2步骤,最后将磁珠用保存液保存,此时磁珠的浓度可自定。 三、羧基磁性微球偶联抗体 1. 偶联条件 1.1. 活化剂:EDC和NHS 1.2. 反应温度:37℃环境 1.3. 反应时间: 1.3.1.活化时间:30min 1.3.2.偶联时间:2h
抗体库的构建 【材料和试剂】 (1)电转仪 (2)电击杯 (3)SB培养基 (4)SOC培养基 SB培养基经高压灭菌后,降温至60℃或60℃以下,加入20ml经过滤除菌的1mol/L葡萄糖溶液。 50mg/ml氨苄青霉素水溶液,过滤除菌。 10mg/ml 卡那霉素水溶液,过滤除菌。 4% PEG溶液 【操作步骤】 (1)将第4步得到的连接物与300
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