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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Ni-NTA purified truncated recombinant BLK expressed in E. Coli strain BL21 (DE3).
- 保存条件:
stored at -20℃, stable for one year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
小鼠
- 应用范围:
E, WB, IHC
- 抗体英文名:
BLK Antibody [9D10D1]
- 抗体名:
BLK Antibody [9D10D1]
- 规格:
0.1mL
BLK 抗体 [9D10D1] ,BLK Antibody [9D10D1] ,BLK 抗体 [9D10D1] ,BLK Antibody [9D10D1]
产品名称:BLK 抗体 [9D10D1] -BLK Antibody [9D10D1]
产品货号:PSI-32-116-0.1mL
产品规格:0.1mL
背景资料:BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking.Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR.
保存建议:BLK 抗体 [9D10D1] -BLK Antibody [9D10D1] stored at -20℃, stable for one year
应用类型:E, WB, IHC
点击:BLK 抗体 [9D10D1] -BLK Antibody [9D10D1] 查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验【摘 要】目的:培养脑皮质微血管内皮细胞。方法:取1~5d Wistar乳鼠脑皮质,经不同孔径的筛网过滤后,用胶原酶振荡消化获得的微血管内皮细胞进行培养,用Ⅷ因子相关抗原免疫组化鉴定。结果:培养的细胞呈单层贴壁生长,7~9d呈典型的铺路卵石样征象。结论:建立了一种简便易行的培养脑皮质微血管内皮细胞的方法。【关键词】 细胞培养;微血管内皮细胞;Wistar大鼠脑微血管内皮细胞是构成血脑屏障的主要成分,具有特殊的形态结构和机能,在许多病理状态下起重要作用[1]。建立脑微血管内皮细胞体外培养,可获
Dual- and Triple-Color Fluorospot
in several publications and reagents/kits for various cytokine combinations and species are commercially available. The most common protocol for fluorospot includes the use of biotinylated detection antibodies for one cytokine and an FITC-labeled detection antibody
1.材料和方法1.1 DMEM/F12条件培养液(亚硒酸钠40μg·L-1,腐胺16.2 mg·L-1,转铁蛋白100 mg·L-1,胰岛素234U·L-1,甲状腺素0.4μg·L-1,黄体酮0.62 mg·L-1,谷氨酰胺3g·L-1)。DMEM/F12培养基、小牛血清购自Hyclone公司,兔抗鼠GC抗体、亚硒酸钠、腐胺等购自Sigma公司。1.2 视神经胶质细胞的来源、培养 取新生2d的Wistar大鼠10只(第三军医大学大坪医院野战外科研究所动物中心提供),无菌条件下取出双
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