Mouse IgG3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IgG3 in the samples. A monoclonal antibody specific for mouse IgG3 has been pre-coated onto a microplate. Standardsand samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for IgG3 is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IgG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.