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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of mouse BAF250 (NP_001074288.1).
- 保存条件:
Store at -20℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
WB
- 抗体英文名:
BAF250 Antibody
- 抗体名:
BAF250 Antibody
- 规格:
50uL
BAF250 抗体,BAF250 Antibody,BAF250 抗体,BAF250 Antibody
产品名称:BAF250 抗体-BAF250 Antibody
产品货号:PSI-16-694-50uL
产品规格:50uL
背景资料:This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.
保存建议:BAF250 抗体-BAF250 AntibodyStore at -20℃
应用类型:WB
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文献和实验问:BAF cell的来源是什么?有什么特性和用途呢?答:ATCC上没有你所谓的BAF cell,DSMZ上有两种BAF cell,如下:Cell line:BA-F8Cell type:mouse hybridoma(anti-rat fiber/myosin)Origin:this hybridoma cell line was described to secrete a mouse monoclonal antibody(IgG2b)that reacts with rat type
Monoclonal Antibody Production
) (3) 250 ml bottles of 100X penicillin-streptomycin (4) 10 ml of 100X HAT selection solution (5) 10 ml of 100X HT solution (6) 10 sterile flat-bottomed 96-well plates 3. Assaying for Positive Clones Run an ELISA.html">indirect ELISA using
Polyclonal Antibody Production
from the muscle, insert the needle 1-2 cm at a shallow angle so as to not hit muscle, and inject 250 µL of your innoculum. After the required volume has been injected, let the needle sit for a few seclonds, then pull out and gently rub the injection site
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