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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human PRKAB1 (NP_006244.2).
- 保存条件:
Store at -20℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
WB,IHC,IF
- 抗体英文名:
PRKAB1 Antibody, KO Validated
- 抗体名:
PRKAB1 Antibody, KO Validated
- 规格:
50uL
PRKAB1 抗体, KO Validated,PRKAB1 Antibody, KO Validated,PRKAB1 抗体, KO Validated,PRKAB1 Antibody, KO Validated
产品名称:PRKAB1 抗体, KO Validated-PRKAB1 Antibody, KO Validated
产品货号:PSI-14-458-50uL
产品规格:50uL
背景资料:The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
保存建议:PRKAB1 抗体, KO Validated-PRKAB1 Antibody, KO ValidatedStore at -20℃
应用类型:WB,IHC,IF
点击:PRKAB1 抗体, KO Validated-PRKAB1 Antibody, KO Validated查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验Methods 2016 年的文章2,国际抗体验证工作小组(International Working Group on Antibody Validation, IWGAV)提出 5 种支柱策略(conceptual pillar),如下:1. 基因策略(敲除验证):利用 CRISPR/Cas 或 RNAi 等技术对靶基因进行敲除(knock out)或敲低(knock down),分别检测对照组与 KO 组的信号。2. 正交策略:在多种样品中使用一种与抗体无关的定量方法,然后比较分析基于抗体的定量
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Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA
Hyperphage is a substitute for M13KO7 helper phage used in antibody phage display. It allows to improve the antibody display efficiency on phage by 2-3 orders of magnitude. This is achieved by forcing the packaging E. coli cell to exclusively
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