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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
IL-1RAcP antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-1RAcP.<br>The immunogen is located within the last 50 amino acids of IL-1RAcP.
- 保存条件:
stored at 4℃ for three months and -20℃, stable for up to one year
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
E, WB, ICC, IF
- 抗体英文名:
IL-1RAcP Antibody
- 抗体名:
IL-1RAcP Antibody
- 规格:
0.02mg
IL-1RAcP 抗体,IL-1RAcP Antibody,IL-1RAcP 抗体,IL-1RAcP Antibody
产品名称:IL-1RAcP 抗体-IL-1RAcP Antibody
产品货号:PSI-2129-0.02mg
产品规格:0.02mg
背景资料:IL-1RAcP Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-κB inducing kinae (NIK) and of NF-κB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
保存建议:IL-1RAcP 抗体-IL-1RAcP Antibody stored at 4℃ for three months and -20℃, stable for up to one year
应用类型:E, WB, ICC, IF
点击:IL-1RAcP 抗体-IL-1RAcP Antibody查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验; TIMP, tissue inhibitor of metalloproteinase; TNF, tumor necrosis factor. IL-33 signaling occurs via the ST2L receptor. Binding of IL-33 to ST2L induces the recruitment of IL-1RAcP and activation of intracellular signaling pathways including
或尼日利亚菌素可以有效的刺激活化的 IL-1β 的表达。可综合已有文献及预实验来摸索找到合适的条件。3. 实验步骤基于上述提及的靶标的特点,那么检测 IL-1β 时除了按照 CST 推荐的抗体使用方法外,还需注意一些细节。细胞培养基样本的制备活化的 IL-1β 主要分泌到细胞培养基中,那么需要用丙酮沉淀蛋白。方法如下:1) 取一份体积培养基和 4 份体积的冷的丙酮混合, -80 度下过夜。2) 10,000 g 离心 10 分钟。3) 小心去除上清,期间防止取到蛋白沉淀。不要使沉淀干燥过度,否则后续不容
of metalloproteinase; TNF, tumor necrosis factor. IL-33 signaling occurs via the ST2L receptor. Binding of IL-33 to ST2L induces the recruitment of IL-1RAcP and activation of intraCell ular signaling pathways including MyD88 and TRAF6-dependent activation
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