M-CSF Protein, Mouse, Recombin

蛋白长度：A DNA sequence encoding the mouse CSF1 (P07141-1) (Met1-Glu262) was expressed.

性质：

表达系统：HEK293 Cells

种属：Mouse

氨基酸序：Met1-Glu262

标签类型：No tag

分子量：The recombinant mouse CSF1 comprises 230 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of the protein is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.

活性：1. Immobilized mouse CSF1 (Cat: PM52813M0) at 2 μg/ml (100 μl/well) can bind mouse CSF1R-Fch (Cat: 50059-M03H), The ECof mouse CSF1R-Fch (Cat: 50059-M03H) is 60-220 ng/mL. 2. Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The EDfor this effect is typically 3-15 ng/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：colony stimulating factor 1 (macrophage)

通用名：CSF1

蛋白序参考：

总结：Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：C87615 Protein, Mouse; Csfm Protein, Mouse; MCSF Protein, Mouse; op Protein, Mouse