2B4/CD244 Protein, Human, Reco

蛋白长度：A DNA sequence encoding the human CD244 (NP_057466.1) (Met1-Arg221) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Arg221

标签类型：C-hFc & AVI

分子量：The recombinant human CD244 consists of 453 amino acids and predicts a molecular mass of 50.80 kDa. It migrates as an approximately 65.58 kDa band in SDS-PAGE under reducing conditions.

活性：Measured by its binding ability in a functional ELISA. Immobilized Human CD48 hFc(Cat: PH52003M4) at 2 μg/mL (100 μL/well) can bind Human 2B4/CD244 (hFc & AVI Tag), Biotinylated(Cat: PH52239M4), the ECof Human 2B4/CD244 (hFc & AVI Tag), Biotinylated(Cat:PH52239M4) is 10-70 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：CD244 molecule, natural killer cell receptor 2B4

通用名：CD244

蛋白序参考：NP_057466.1

总结：The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：2B4 Protein, Human; NAIL Protein, Human; NKR2B4 Protein, Human; Nmrk Protein, Human; SLAMF4 Protein, Human