IL36RN/IL-1F5 Protein, Human,

蛋白长度：A DNA sequence encoding the mature form of human IL1F5 (Q9UBH0) (Met1-Asp155) was expressed.

性质：

表达系统：E. coli

种属：Human

氨基酸序：Met1-Asp155

标签类型：No tag

分子量：The recombinant human IL1F5 consists of 156 amino acids and predicts a molecular mass of 17.1 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.

活性：Measured by its ability to inhibit IL36α-induced IL8 secretion in A431 human epithelial carcinoma cells. The EDfor this effect is typically 0.1-0.4μg/mL in the presence of 20ng/mL IL36α.

内毒素：Please contact us for more information.

基因名称：interleukin 36 receptor antagonist

通用名：IL36RN

蛋白序参考：

总结：Interleukin-1 family member 5 (IL-1F5), also known as interleukin 36 receptor antagonist (IL36RA), is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). IL-1F5 is a highly and specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist, and interleukin-1 receptor type I (IL-1R1), which is present in epithelial barriers and takes part in the local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor, single Ig IL-1R-related molecule (SIGIRR)/TIR8, as the effects were not observed in SIGIRR-/- mice. In contrast to its effects in brain tissue, IL-1F5 did not attenuate LPS-induced changes, or up-regulated IL-4 in macrophages or dendritic cells, suggesting that the effect is confined to the brain.

形式：Lyophilized

纯度：> 99 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：FIL1 Protein, Human; FIL1(DELTA) Protein, Human; FIL1D Protein, Human; IL-36Ra Protein, Human; IL1F5 Protein, Human; IL1HY1 Protein, Human; IL1L1 Protein, Human; IL1RP3 Protein, Human; IL36RA Protein, Human; IL36RN Protein, Human; PSORP Protein, Human; PSORS14 Protein, Human