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- 详细信息
- 技术资料
- 保存条件：
Stable for 1 year at -1580°C or below from date of shipment.
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
50 ug
蛋白长度：A DNA sequence encoding the amino acid sequence (Met 1-Leu 878) of human PRKD2 (NP_057541.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
性质：
表达系统：Baculovirus-Insect Cells
种属：Human
氨基酸序：Met1-Leu878
标签类型：N-GST & His
分子量：The recombinant human PRKD2/GST chimera consists of 1115 amino acids and has a calculated molecular mass of 124 kDa. It migrates as an approximately 120 kDa band in SDS-PAGE under reducing conditions.
活性：The specific activity was determined to be ＞30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称：protein kinase D2
通用名：PRKD2
蛋白序参考：NP_057541.2
总结：Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein that belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-71 and phosphorylation of Ser-76 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
形式：Liquid
纯度：> 82 % as determined by SDS-PAGE.
储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Supplied as sterile 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0 Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
别名：HSPC187 Protein, Human; nPKC-D2 Protein, Human; PKD2 Protein, Human
性质：
表达系统：Baculovirus-Insect Cells
种属：Human
氨基酸序：Met1-Leu878
标签类型：N-GST & His
分子量：The recombinant human PRKD2/GST chimera consists of 1115 amino acids and has a calculated molecular mass of 124 kDa. It migrates as an approximately 120 kDa band in SDS-PAGE under reducing conditions.
活性：The specific activity was determined to be ＞30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称：protein kinase D2
通用名：PRKD2
蛋白序参考：NP_057541.2
总结：Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein that belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-71 and phosphorylation of Ser-76 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
形式：Liquid
纯度：> 82 % as determined by SDS-PAGE.
储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Supplied as sterile 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0 Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
别名：HSPC187 Protein, Human; nPKC-D2 Protein, Human; PKD2 Protein, Human
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Protein Kinase D2/PRKD2 Protein, Human, Recombinant (His & GST Tag)
$3215
