CD3D & CD3E Heterodimer Protei

蛋白长度：A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ala105 & Met1-Asp126

标签类型：C-Flag & His

分子量：The recombinant heterodimer of human CD3D&CD3E comprises 271 (126+145) amino acids and has a calculated molecular mass of 31.05 (14.4+16.65)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.

活性：Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein (Cat: PH51482M9) at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a (Cat: 10977-M001). The ECof OKT3-mIgG2a (Cat: 10977-M001) is 7-28 ng/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：CD3d molecule, delta (CD3-TCR complex)

通用名：CD3D & CD3E

蛋白序参考：

总结：

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CD3-DELTA Protein, Human; IMD19 Protein, Human; T3D Protein, Human