HER3/ERBB3 Protein, Human, Rec

蛋白长度：A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Pro730-Ser1065

标签类型：N-GST & His

分子量：The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions.

活性：1. No Kinase Activity2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST (Cat:PH50510I6) bound to human NRG1 (aa 2-224) (Cat:11609-H04H) was 20 nM.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：erb-b2 receptor tyrosine kinase 3

通用名：ERBB3

蛋白序参考：NP_001973.2

总结：ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

形式：Lyophilized

纯度：> 75 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% glycerol, 1mM GSH Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：c-erbB-3 Protein, Human; c-erbB3 Protein, Human; EEBB3 Protein, Human; ErbB-3 Protein, Human; erbB3-S Protein, Human; HER3 Protein, Human; LCCS2 Protein, Human; MDA-BF-1 Protein, Human; p180-ErbB3 Protein, Human; p45-sErbB3 Protein, Human; p85-sErbB3 Protein, Human