Orange fluorescent protein Protein, Discosoma sp, Recombinant (His Tag)

Orange fluorescent protein Pro

tein, Discosoma sp, Recombinant (His Tag)
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  • $3865
  • Leading Biology
  • 美国
  • PO55475E2
  • 2025年07月05日
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    • 详细信息
    • 技术资料
    • 保存条件

      Stable for 1 year at -5495°C or below from date of shipment.

    • 保质期

      1-2年

    • 供应商

      安诺伦(北京)生物科技有限公司

    • 规格

      50 ug

    蛋白长度:OFPSparkTM is a red (orange) fluorescent protein derived from DsRed(similarity is 98%). OFPSparkTM was expressed with a polyhistide tag at the C-terminus(Patent 201510003374.3).

    性质:

    表达系统:E. coli

    种属:Discosoma sp

    氨基酸序:

    标签类型:C-His

    分子量:The recombinant Discosoma sp. OFPSparkTM consisting of 231 amino acids and has a calculated molecular mass of 26.4 kDa.

    活性:OFPSparkTM expression vector transfected 293 H cells transiently. After 48 h, the strong orange fluorescent signals can be detected under the excitation channel of 503.5~547.5 nm, and the strong red fluorescent signals can be detected under the excitation channel of 532.5~587.5 nm.

    内毒素:Please contact us for more information.

    基因名称:

    通用名:OFP

    蛋白序参考:

    总结:OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. Fast OFPSparkTM maturation makes it detectable in mammalian cells as early as within 8 hrs after transfection. OFPSparkTM can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSparkTM expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSparkTM performs well in some fusions and protein labeling applications.

    形式:Lyophilized

    纯度:> 95 % as determined by SDS-PAGE.

    储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

    储存溶液:Lyophilized from sterile Tris, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

    别名:OFP Protein, Discosoma sp

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