EphA2 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human EPHA2 (NP_004422.2) (Met1-Asn534) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Asn534

标签类型：C-AVI & His

分子量：The recombinant human EPHA2 consists of 537 amino acids and predicts a molecular mass of 59.4 kDa.

活性：Measured by its binding ability in a functional ELISA. Immobilized EFNA1-Fch (Cat:10882-H03H) at 10 μg/mL (100 μL/well) can bind Human EphA2, Biotinylated (Cat:PH50506M9), the ECof Human EphA2, Biotinylated (Cat:PH50506M9) is 20-40 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：EPH receptor A2

通用名：EphA2

蛋白序参考：NP_004422.2

总结：Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：ARCC2 Protein, Human; CTPA Protein, Human; CTPP1 Protein, Human; CTRCT6 Protein, Human; ECK Protein, Human