TNFR-2 / CD120b Protein, Human

蛋白长度：A DNA sequence encoding the extracellular domain (Met1-Asp257) of human TNFRII (NP_001057.1) was expressed with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Asp257

标签类型：C-His

分子量：The recombinant human TNFRII comprises 246 amino acids and has a calculated molecular mass of 26.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII is approximately 40.1 kDa due to glycosylation.

活性：1. Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EDfor this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α. 2. Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha (Cat:PH51747E0) at 2 μg/ml (100 μl/well) can bind Human TNFR2/TNFRSF1B His(Cat:PH50451M2), the ECof Human TNFR2/TNFRSF1B His is 2.0-12.0 ng/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：tumor necrosis factor receptor superfamily, member 1b

通用名：TNFRSF1B

蛋白序参考：NP_001057.1

总结：Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

形式：Lyophilized

纯度：> 97 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CD120b Protein, Human; p75 Protein, Human; p75TNFR Protein, Human; TBPII Protein, Human; TNF-R-II Protein, Human; TNF-R75 Protein, Human; TNFBR Protein, Human; TNFR1B Protein, Human; TNFR2 Protein, Human; TNFR80 Protein, Human