- ￥680 - 1280
- KEMOBio
- 进口/国产
- KM103056
- 2025年07月12日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃
- 保质期：
90天
- 英文名：
sgRNA(MS2) cloning backbone
- 库存：
100
- 供应商：
科淼生物
- 规格：
0.5ug/0.5ug
| 规格： | 0.5ug | 产品价格： | ￥680.0 |
|---|---|---|---|
| 规格： | 0.5ug | 产品价格： | ￥1280.0 |
英文名称：sgRNA(MS2) cloning backbone
货号：KM103056
图谱：微客服
宿主：哺乳细胞
用途：基因敲除
片段类型：
片段物种：
原核抗性：Amp
真核抗性：
荧光标记：
其他产品推荐：
pEnCMV-EGFR L858R-3×FLAG(2同义突变)
pCMV-SPORT6-Slc22a13(mouse)
pDNR-LIB-XAGE3(human)
pCMV-Nfatc2(mouse)-3×FLAG-Neo
pCMV-SPORT6-GGTLC1(human)
pCMV-SPORT6-MRE11
pCMV-6×His-Ptpn13(mouse)-Neo
pDNR-LIB-LSM5(1同义突变)(human)
pPGK-Fluc-PHLPP2(human)-3UTR-mut3-hRluc
pOTB7-MTCH1
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验backbone are run side by side,preferably beside a known amount of cut DNA.You can then use this to estimate how much backbone and insert to use in your ligation reaction.In general,a large excess of insert over backbone works well,but you should try
Cloning Strategies for Catalytic Antisense RNAs
to their target RNA in the same manner as conventional antisense RNAs, and as a second element of their suppressive function, they induce chemical cleavage of the phosphodiester backbone of their target RNA by the same mechanism as ribozymes. Thus, the main
as described in the cloning manual to make sure it is free of DNAse. 7)Preparation of backbone:I have never gel purified a cut backbone,although some people have success doing this.What I found easy,and which worked,was to cut a known amount of backbone (e.g
技术资料
