Zorbax XDB-C18,4.6x15mm,1.8um,

以非那西丁为探针采用高效液相色谱法快速测定细胞色素P450 1A2的酶活性

　　【摘要】  目的建立一种快速、高效的以非那西丁作为探针药物评价细胞色素P450 1A2（CYP1A2）酶活性的高效液相色谱-紫外检测方法。方法色谱柱为Agilent Zorbax SB-C18柱（50 mm×4.6 mm I.D.，5 μm）；流动相为乙腈-水（含0.01％七氟丁酸），梯度洗脱，流速2.0 ml・min-1；检测波长为244 nm。非那西丁与人CYP1A2酶在37 ℃温孵适当时间后，加入50 μl乙腈终止反应，10 000 g离心后取上清液进样分析测定。结果对乙酰

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

., Zorbax XDB, Agilent Technologies) CAUTION: Do not inhale; use in a chemical fume hood. C18 solid-phase extraction pipette tips (e.g., SPEC Plus PT C18, ANSYS Technologies) These C 18 solid-phase disk pipette tips have a 0.4-µg sorbent

Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism

3 × 250–mm, 3.5‐μm particle size C18‐SB reverse‐phase column (Zorbax, Agilent) 4.6 × 12–mm, 5‐μm particle size C18‐SB guard column (Zorbax, Agilaent