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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD117 (c-Kit)
- 级别:
CD117 (c-Kit) Antibody
- 目录编号:
CD117 (c-Kit) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD117 (c-Kit) Antibody
- 抗体英文名:
Dazzle™ 594 anti-mouse CD117 (c-Kit) Antibody-PE
- 抗体名:
CD117 (c-Kit) Antibody
- 标记物:
PE
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD117 (c-Kit) Antibody
- 亚型:
CD117 (c-Kit) Antibody
- 形态:
CD117 (c-Kit) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2400.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cellfactor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kitligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 isexpressed on hematopoietic stem cells (including multipotent hematopoietic stem cells,progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mastcells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for thedevelopment of hematopoietic stem cells.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Mouse bone marrow mast cells
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
2. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
中。5. 用无菌 PBS 将上述锥形试管填满(至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下,以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞(BD 产品目录号:555899,10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。)8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中(PBS 含有 5% 正常人血清)。9. 加入抗体:CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
荧光二抗的选择――Dylight荧光与传统荧光标记的比较 顾名思义,荧光二抗 通常指代的就是带荧光团标记的二抗,这样的二抗可以通过观察其在不同波长下荧光的强度来确定其二抗的含量。常见的荧光团包括FITC、Rhodamine、Texas Red、PE、Cy系列等。比如FITC即异硫*酸荧光素,是目前应用最广泛的荧光素。最大吸收光波长为490~495nm,最大发射光波长为 520~530nm,呈现明亮的黄绿色荧光。其优势是分子量较小为389.4,每一个抗体可标记几个










