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文献和实验CellTrace CFSE Cell Proliferation Kit
after hydrolysis are 492 nm and 517 nm, respectively. Cells labeled with CellTrace™ CFSE can be visualized by fluorescence microscopy using standard fluorescein filter sets or analyzed by flow cytometry in an instrument equipped with a 488 nm excitation source
LIVE/DEAD® Violet Viability/Vitality Kit
8. Analyze the stained cells by flow cytometry using violet (~405 nm) excitation and violet fluorescence emission (~450 nm) for the calcein violet (live cells) and blue-green fluorescence emission (~525 nm) for the aqua-fluorescent reactive dye
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
a standard alkaline phosphatase staining kit to reveal expression of the pluripotent stem cell marker APLP. Note that while hCD29 is expressed by all human pluripotent stem cells throughout each colony (E), this antibody fails to stain live cells
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