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文献和实验CellTrace CFSE Cell Proliferation Kit
after hydrolysis are 492 nm and 517 nm, respectively. Cells labeled with CellTrace™ CFSE can be visualized by fluorescence microscopy using standard fluorescein filter sets or analyzed by flow cytometry in an instrument equipped with a 488 nm excitation source
LIVE/DEAD® Violet Viability/Vitality Kit
8. Analyze the stained cells by flow cytometry using violet (~405 nm) excitation and violet fluorescence emission (~450 nm) for the calcein violet (live cells) and blue-green fluorescence emission (~525 nm) for the aqua-fluorescent reactive dye
, where tissue autofluorescence is minimal. Here we describe the manufacture of 12-nm 3-dimercaptosuccinic acid-coated Fe3 O4 magnetic nanoparticles, their labeling with a new near-infrared fluorophore, IRDye800CW (excitation/emission: 778/806 nm
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