GoInVivo™ Purified 抗小鼠 CD279 (

GoInVivo™ Purified anti-mouse CD279 (PD-1) Antibody详细如下：

Description ：CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging tothe CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells andthymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyteclonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positiveselection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation isessential for prolonged survival of intratesticular islet allografts.

Verified Reactivity ：Mouse

Antibody Type ：Monoclonal

Host Species ：Rat

Immunogen ：PD-1 cDNA followed by PD-1-Ig fusion protein

Formulation ：0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxinlevel is <1.0 EU/mg of the protein. (<0.01 pg/µg of the protein) as determined by the LAL test.

Preparation ：The GoInVivo™ antibody was purified by affinity chromatography.

Concentration ：The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and3 mg/mL.

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solutioncontains no preservative; handle under aseptic conditions.

Application ：FC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimalperformance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment todetermine the optimal concentration to use for each particular experiment.