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文献和实验Protocol for First-strand cDNA Synthesis
the mix at 70℃for 5 minutes and chill on ice.Add the following in the order indicated:5X reaction buffer 4µl,10mM 4 dNTP mix 2µl (1.0mM - final concentration),ribonuclease inhibitor 20u,deionized water (nuclease free) to 19µl.Incubate at 37℃for 5 minutes
of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
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