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常温
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5年
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- 供应商:
维百奥生物
CSM-Ura Powder, 100 grams
CATALOG #: 1004-100
Recipe in mg/L:
AMINO ACIDS & SUPPLEMENTS
Adenine hemisulfate (10 mg/L)
L-Arginine (50 mg/L)
L-Aspartic acid (80 mg/L)
L-Histidine hydrochloride monohydrate (20 mg/L)
L-Isoleucine (50 mg/L)
L-Leucine (100 mg/L)
L-Lysine hydrochloride (50 mg/L)
L-Methionine (20 mg/L)
L-Phenylalanine (50 mg/L)
L-Threonine (100 mg/L)
L-Tryptophan (50 mg/L)
L-Tyrosine (50 mg/L)
L-Valine (140 mg/L)
Instructions
Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with
stirring for 10-15minutes, and filter sterilize or autoclave at 121°C for 15 minutes.
Suggested g/L: 0.77
Storage Temperature: Room Temp
Related Categories
YNB (Yeast Nitrogen Base), Replica Plating, Enzymatic Lysis of Yeast
CATALOG #: 1004-100
Recipe in mg/L:
AMINO ACIDS & SUPPLEMENTS
Adenine hemisulfate (10 mg/L)
L-Arginine (50 mg/L)
L-Aspartic acid (80 mg/L)
L-Histidine hydrochloride monohydrate (20 mg/L)
L-Isoleucine (50 mg/L)
L-Leucine (100 mg/L)
L-Lysine hydrochloride (50 mg/L)
L-Methionine (20 mg/L)
L-Phenylalanine (50 mg/L)
L-Threonine (100 mg/L)
L-Tryptophan (50 mg/L)
L-Tyrosine (50 mg/L)
L-Valine (140 mg/L)
Instructions
Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with
stirring for 10-15minutes, and filter sterilize or autoclave at 121°C for 15 minutes.
Suggested g/L: 0.77
Storage Temperature: Room Temp
Related Categories
YNB (Yeast Nitrogen Base), Replica Plating, Enzymatic Lysis of Yeast
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文献和实验相关实验
Fermentor worm chow Prep Have autoclaved: 250 ml straight neck flask 100 mmfunnel Fermentor vessel with all openings covered, top loosened, on its side in tub with wedges, after a/c sit upright
ml。 室温保存1-2个月。 **也可以所有组分一起灭菌,但是可能会发生焦化作用,然而焦化作用不会引起任何问题。 生长选择合成培养基(Selective Growth Synthetic Media) 2x (SD – CAA) 1)用于EBY100菌株配套 pYD 系列载体 ,或者二配体酵母配套pPNL20 和pPNL30 载体 10 g/L 酪蛋白水解物casamino acids (-ade, -ura, -trp
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Sunrise 货号1004-100 CSM-Ura Powder,100 grams
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