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文献和实验Preparing a Selenomethionyl Protein
filtered) 2M CaCl2 (sterile filtered) 0.5% (w/v) Thiamine Solution (sterile filtered) Procedure Day 1 Prepare a 2mL day culture consisting of 2mL LB media, 2uL antibiotics (1000x conc.), and a single E.coli colony. Grow at 37
Protein purification; actin Overview ACTIN The most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various isoforms; structure resolved at 2.8 Angstrom. [see also protocol ID 207,208,209]
Protein Expression and Purification Protocol
to appear. Step 2: Make a starter culture for protein expression. Usually to about 250 to 500 mL of LB broth the antibiotic is added. a) Generally, the antibiotic is ampicillin, which is added to the LB broth at 100 m g/ml final concentration
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