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文献和实验TRICINE GELS Protocol (For Low MW proteins (
ml 6.70 ml 10% APS 133 µl 66 µl 160 µl 80 µl TEMED 13.2 µl
) 1-8μl Calf Thymus DNA (0.5ug/ul) 2μl TE buffer 0-7μl Total Volume 10μl Add 1ul of 4% Formic Acid and incubate for 25 min at 37℃. During this incubation, add 15 μl of stock piperidine to 135 μl
, for their effects on fungal disease development. The protocols for carrot transformation with a thaumatin-like protein are described. In addition, the use of herbicide resistance as a selectable marker in carrot transformation is illustrated. In this protocol
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