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- 154707
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- 2025年07月15日
- PG
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD204 (Msr1)
- 级别：
CD204 (Msr1) Antibody
- 目录编号：
CD204 (Msr1) Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD204 (Msr1) Antibody
- 抗体英文名：
TotalSeq™-C0448 anti-mouse CD204 (Msr1) Antibody
- 抗体名：
CD204 (Msr1) Antibody
- 标记物：
CD204 (Msr1) Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD204 (Msr1) Antibody
- 亚型：
CD204 (Msr1) Antibody
- 形态：
CD204 (Msr1) Antibody
- 应用范围：
PG
- 保存条件：
2-8℃
- 浓度：
0.5 mg/mL
- 规格：
10 ug
Description ：CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor(MSR), is 220 kDa, trimeric type II transmembrane protein, with one scavenger receptorcysteine-rich domain (SRCR). It is a phagocytic pattern-recognition receptor (PRR) expressedon macrophages and dendritic cells. CD204 is a receptor mediating recognition andinternalization of low-density lipoprotein (LDL) by macrophages and plays a critical role inatherogenesis. CD204 also recognizes apoptotic cells, modified lipid proteins, and exogenouspathogen-associated molecular patterns (PAMPs), which results in the induction of innateimmune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors,such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines.CD204 has been implicated in several pathological processes such as Alzheimer’s disease,sepsis, ischemic injury, and coronary artery disease.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Recombinant mouse CD204 extracellular domain
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration ：0.5 mg/mL
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
沉淀和聚集，影响抗体的效价，随着先进技术的出现，冻干粉形式的抗体已经上市，如美天旎品牌的StainExpress™系列抗体可以在19−25°C环境下保存。 抗体选择 大多数只标明用于WB、IP、IHC、IF、ChIP的抗体都不适用于流式实验(FC,flowcytometry)。选择标明用于FC实验的抗体，在Panel设计上咨询专业人士，通过自己的实验结果进行改进，采用专业的工具进行配色辅助，文献和实验数据图作为参考。
有好几个同仁发消息问CD31染色，刚工作比较忙，今天分享一下我做的是免疫荧光，也试做过免疫组化，都可以做出来抗体：小鼠抗CD31单克隆抗体 英国Abcam公司（CD31抗体说明书里面有说多聚甲醛会掩盖抗体，所以推荐速冻切片，也尝试过速冻切片，不使用多聚甲醛，但是这个技术个人掌握不好，所以还是用了多聚甲醛固定）二抗： 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明：我是从同学那里得到的经验，可能绿光稍微好做些，之前做红光
