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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD107a (LAMP-1)
- 级别：
CD107a (LAMP-1) Antibody
- 目录编号：
CD107a (LAMP-1) Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD107a (LAMP-1) Antibody
- 抗体英文名：
TotalSeq™-C0905 anti-mouse CD107a (LAMP-1) Antibody
- 抗体名：
CD107a (LAMP-1) Antibody
- 标记物：
CD107a (LAMP-1) Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD107a (LAMP-1) Antibody
- 亚型：
CD107a (LAMP-1) Antibody
- 形态：
CD107a (LAMP-1) Antibody
- 应用范围：
PG
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
10 µg
Description ：CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation,CD107a is transferred to the cell membrane surface of activated platelets, activatedlymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107ahas been suggested to play a role in the protection of lysosomal membrane from lysosomalhydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediatesautoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surfaceexpression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culturecell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
沉淀和聚集，影响抗体的效价，随着先进技术的出现，冻干粉形式的抗体已经上市，如美天旎品牌的StainExpress™系列抗体可以在19−25°C环境下保存。 抗体选择 大多数只标明用于WB、IP、IHC、IF、ChIP的抗体都不适用于流式实验(FC,flowcytometry)。选择标明用于FC实验的抗体，在Panel设计上咨询专业人士，通过自己的实验结果进行改进，采用专业的工具进行配色辅助，文献和实验数据图作为参考。
CD63 分子 CD63 常用单克隆抗体或代号： RUU- SP2.28,CLB 主要表达细胞： Pt,M,Mac,(G,T,B) [Pt] 分子质量(kDa)和结构： gp53,血小板颗粒蛋白(TM4- SF) 功 能： 血小板活化,中性粒细胞- 活化内皮细胞粘附 CD63 Aka lysosomal-membrane-associated glycoprotein 3, LAMP
